Figure 5
From: Reduced background autofluorescence for cell imaging using nanodiamonds and lanthanide chelates
The signal intensity of cells on the detected channel is illustrated. The average intensity of 10 cells on each slide is presented, where error bars indicate the standard deviation of the 10 measured cells. The average brightness of cells in which E-selectin expression was induced with TNFα are colored blue, and those which were not induced to express E-selectin are colored red. (a) 30 nm FND-PEG-SA used with monovalent SLeX-biotin and multivalent SLeX-PAA-biotin, compared to the antibody-functionalised FNDs of the same size. (b) 100 nm FND-PEG-SA used with monovalent SLeX-biotin and multivalent SLeX-PAA-biotin, compared to the antibody functionalised nanodiamonds. (c) SA-BHHTEGST-Eu used with monovalent SLeX-biotin and multivalent SLeX-PAA-biotin.
