Table 2 The brightness of cells containing 30 nm FND-PEG-SA, 100 nm FND-PEG-SA, and SA-BHHTEGST-Eu complexed to E-selectin ligands SLeX-biotin or SLeX-PAA-biotin, in Arbitrary Units (AU) is listed. The brightness of cells containing 30 nm and 100 nm FND-PEG-E-selectin antibody (Ab) are also shown.

From: Reduced background autofluorescence for cell imaging using nanodiamonds and lanthanide chelates

 

30 nm FND-PEG

100 nm FND-PEG

SA-BHHTEGST-Eu

SA/SLeX-Biotin

SA/SLeX-PAA-Biotin

Ab

SA/SLeX-Biotin

SA/SLeX-PAA-Biotin

Ab

SLeX-Biotin

SLeX-PAA-Biotin

TNFα

27.6 ± 11.5

15.3 ± 7.6

16.0 ± 8.8

11.0 ± 7.0

13.7 ± 4.0

15.9 ± 8.1

10.4 ± 3.8

20.6 ± 9.5

No TNFα

10.9 ± 4.7

6.0 ± 4.7

0.4 ± 0.5

2.6 ± 3.9

5.2 ± 4.9

8.1 ± 2.3

7.5 ± 2.9

3.8 ± 1.5

TNFα/No TNFα

2.5

2.6

40

4.2

2.6

2.0

1.4

5.4

  1. The average cell brightness intensity is presented (±standard deviation). The average background noise intensity was 0.04 for 30 nm FND-PEG-SA, 0.10 for 100 nm FND-PEG-SA, 1.28 for SA-BHHTEGST-Eu, and 0.01 for FND-PEG-Ab.
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