Figure 4
From: Selection of reference genes for miRNA qRT-PCR under abiotic stress in grapevine

Determination of the optimal number of candidate reference genes by geNorm analysis. Pairwise variation (Vn/n + 1) between the normalization factors NFn and NFn+1 was used to determine the optimal number of multiple reference genes. When the value of Vn/n + 1 is ≤0.15, the addition of one or more reference genes into the reference gene combination would not be necessary.