Table 2 The primers and amplification characteristics of candidate reference genes.
From: Selection of reference genes for miRNA qRT-PCR under abiotic stress in grapevine
Gene | Gene code | Forward primer (5′-3′) | Reverse primer (5′-3′) | Efficiency | R2 |
|---|---|---|---|---|---|
5.8S rRNA | KT344661.1 | GCTCTCGCATCGATGAAGAAC | AGACTCGATGATTCACGGGATT | 2.03 | 0.997 |
U6 snRNA | u6wg_chr6_15577690_15577792_+ | CCGATAAAATTGGAACGATACAGAG | TCGATTTGTGCGTGTCATCCT | 2.13 | 0.995 |
ACT | EC969944 | GACAATGGATGGACCAGATTCA | CTTGCATCCCTCAGCACCTT | 1.99 | 0.997 |
UBQ | EC929411 | GTGGGTGCAGACGTGCATAA | CCTTGATGCAATTGGCTAGGA | 2.14 | 0.991 |
GAPDH | CB973647.1 | AGCCCTCAACGAGAAGTTCTTG | TCGATCACACGGGAGCTGTA | 1.97 | 0.995 |
EF1 | EC959059 | AACCGAAAGCACCTCGATCA | TGGTTGAGTCCTTTGCTTTTCC | 1.98 | 0.999 |
miR160e | MIMAT0005656 | GCGGCGGTGCCTGGCTCC | GTGCAGGGTCCGAGGT | 2.10 | 0.982 |
miR164a | MIMAT0005658 | GCGGCGGTGGAGAAGCAG | GTGCAGGGTCCGAGGT | 2.05 | 0.997 |
miR168 | MIMAT0005675 | GCGGCGGTCGCTTGGTGC | GTGCAGGGTCCGAGGT | 2.17 | 0.996 |
miR156a | MIMAT0005640 | GCGGCGGTGACAGAAGAG | GTGCAGGGTCCGAGGT | 2.09 | 0.977 |
miR159a | MIMAT0005648 | GCGGCGGCTTGGAGTGAA | GTGCAGGGTCCGAGGT | 2.37 | 0.976 |
miR162 | MIMAT0005657 | GCGGCGGTCGATAAACCT | GTGCAGGGTCCGAGGT | 2.57 | 0.989 |
miR167a | MIMAT0005670 | GCGGCGGTGAAGCTGCCA | GTGCAGGGTCCGAGGT | 3.31 | 0.911 |
miR169a | MIMAT0005676 | GCGGCGGCAGCCAAGGAT | GTGCAGGGTCCGAGGT | 2.34 | 0.987 |
miR396a | MIMAT0005724 | GCGGCGGTTCCACAGCTT | GTGCAGGGTCCGAGGT | 2.46 | 0.958 |
