Figure 3
From: Interconnected Microphysiological Systems for Quantitative Biology and Pharmacology Studies
Assessment of MPS functionality in 4-MPS platform. Metrics of tissue function measured during a 2-week co-culture of 4 different MPSs. MPS representing liver, gut, lung, and endometrium were linked using the platform and flow scheme and partitioning shown in Fig. 2. Samples collected from each platform compartment were used to measure protein and metabolite concentrations, which were in turn used to calculate production rates via computational PBPK models. Albumin secretion rates were used as an indicator of liver function (a). Barrier functions of gut (b) and lung (c) MPSs were assessed with TEER measurements. Endometrium MPS functionality was characterized with IGFBP-1 secretion rate to its apical medium (d). Compartment and system volumes are in Table S1. The systemic exchange flow rate Qsys was 5 mL/day.
