Figure 4
From: Interconnected Microphysiological Systems for Quantitative Biology and Pharmacology Studies
Assessment of MPS functionality in 7-MPS platform. Metrics of tissue function measured for 3-week co-culture of 7 different MPS. MPS representing liver, gut, lung, endometrium, heart, pancreas, and brain were interconnected using the platform, and flow scheme and partitioning shown in Fig. 2 with system and individual compartment volumes indicated in Table S1. Samples collected from each platform compartment were used to measure protein and metabolite concentrations, which were in turn used to calculate production rates via computational PBPK models. Albumin secretion rates were used as an indicator of liver function (a). Barrier functions of gut (b) and lung (c) MPSs were assessed with TEER measurements. Endometrium MPS functionality was characterized with IGFBP-1 secretion rate (d) to its apical medium. Heart MPS function was evaluated with beat frequency (e). C-peptide production rates (f) represented pancreas function. N-acetyl aspartate (NAA) concentrations (g) in the apical brain MPS indicated brain MPS functionality. The systemic exchange flow rate Qsys was 10 mL/day.
