Figure 1 | Scientific Reports

Figure 1

From: The development of an extra-anatomic tissue-engineered artery with collateral arteries for therapeutic angiogenesis in ischemic hind limb

Figure 1The alternative text for this image may have been generated using AI.

Nanosized decellularized arteries (NDAs). (A) Biological structures, (1) Gross view, (2) H&E (bar: 200 μm), (3) Masson’s trichrome staining (bar: 200 μm), (4) Van Gieson’s staining (bar: 200 μm). (B) Test, (1) DNA content (P < 0.001), (2) Suture strength (P > 0.05) Fresh arteries (FAs), (3) Burst pressure (P > 0.05), (4) Rehydration (P < 0.001). (C) Microstructure, (1) Cross-section of NDAs (SEM, bar: 50 μm), (2) Intima (SEM, bar: 10 μm), (3) Subintima and media (SEM, bar: 10 μm), (4) Adventitia (SEM, bar: 10 μm). (D) Cell seeding, (1) Immunophenotype of MSCs by flow cytometry, (2) Cytotoxicity (P > 0.05), (3) Cell proliferation on NDAs, (4) MSCs (green) seeded NDAs (H&E, bar: 200 μm), (5) MSCs seeded NDAs (bar: 100 μm), (6) Intima of MSCs seeded NDAs (SEM, bar: 50 μm), (7) Adventitia of MSCs seeded NDAs (SEM, bar: 10 μm).

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