Figure 4
From: The dynamics of TGF-β in dental pulp, odontoblasts and dentin
In vitro activation of latent TGF-β1 by MMP2 or MMP11 and the effect of activated TGF-β1 on gene expression in the PPU-7 cell line. Latent TGF-β1 was incubated with rhMMP2 or rhMMP11. ALP-inducing activity of HPDL cells exposed to (a) latent TGF-β1 only (Latent), latent TGF-β1 with rh-MMP2 (Latent + MMP2) and rh-MMP2 only (MMP2) samples and ALP-inducing activity of HPDL cells exposed to (b) latent TGF-β1 only (Latent), latent TGF-β1 with rh-MMP11 (Latent + MMP11) and rh-MMP11 only (MMP11) samples (n = 6). The mRNA expression (as detected by qPCR) of (c) DSPP-variant 1 (DSPPv1), (d) DSPP-variant 2 (DSPPv2), and (e) MMP20. Each mRNA expression value was normalized to that of the reference gene glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and the relative quantification data for DSPPv1, DSPPv2, and MMP20 in PPU-7 were generated on the basis of a mathematical model for relative quantification in a qPCR system (n = 6). Grey bar graph: PPU-7 without TGF-β1, Black bar graph: PPU-7 with TGF-β1.
