Figure 5
Quantified analysis of proliferation asymmetries in the LVZ and PNE. (A–D) Plots showing asymmetry indexes (AI) in the PNE (A,B) and in the LVZ (C,D), with regard to counts of PH3 (A,C), BrdU (B) and Pax6 (D) positive cells at stages 27 to 31. Dots in (A,C), triangles in (B) and squares in (D) correspond to AIs obtained for individual embryos at stages 27 (yellow), 28 (orange), 29 (blue), 30 (red) and 31 (green). For each group of embryos analysed (same marker, same stage, same zone), horizontal black lines show the median AI value. (E,F) Plots showing Pax6 positive cell counts (E) and ratios (in %) of BrdU to Pax6 positive cell counts (F) in the right and in the left LVZ at stages 29 and 30. Blue and red dots correspond to individual embryos respectively at stage 29 and at stage 30. Best-fit regression lines (blue at stage 29, red at stage 30) are shown, together with the symmetry reference (dotted black line). Light green shading in (A,C–F) with the indication (P < 5E-02) highlight embryo groups exhibiting a significant asymmetry in the distribution of cell counts between the left and the right, based on paired Student’s t-tests on AIs (A,C,D) and ratios of BrdU to Pax6 cell counts (F). In (E), this indication refers to X2 tests conducted on all individual embryos analysed. Abbreviation used: P, p-value.
