Figure 4 | Scientific Reports

Figure 4

From: Bone marrow mesenchymal stem cells protect against n-hexane-induced neuropathy through beclin 1-independent inhibition of autophagy

Figure 4

mTOR-dependent activation of ULK1 contributes the anti-autophagic effects of BMSC. (A) The effects of BMSC transplantation on the levels of ULK1 and p-ULK-1 in the spinal cord and sciatic nerve of HD-intoxicated rats were detected using western blot and the density of blots was quantified (the full-length gels were shown in Supplementary Figure S5A,B). (B) Rapamycin abolished the inhibitory effects of BMSC against HD-induced phosphorylation of ULK1. VSC4.1 cells were treated with HD (25 mM) or saline for 24 h and then were treated with BMSC-CM (15%, v/v) in the presence or absence of Rap for additional 24 h. The levels of ULK1 and p-ULK-1 were determined by Western blot and the density of blots was quantified (the full-length gels were shown in Supplementary Figure S4C). ap < 0.05, compared with control group; bp < 0.05, compared with HD group; cp < 0.05, compared with BMSC-CM group.

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