Figure 1

Intravenous C16 and Ang-1 enhanced the efficacy of PMSC therapy for reducing inflammatory cell infiltration and disease progression in the rat EAE model. (A) Clinical scoring of the severity of EAE symptoms post-injection (pi). n = 10, **P < 0.01 vs. vehicle (PMSCs vs. vehicle: 1 week pi, ES = 1.18, P = 0.28; 2 weeks pi, ES = 8.98, P < 0.0001; 3 weeks pi, ES = 6.72, P = 0.0003; 4 weeks pi, ES = 8.03, P = 0.00023; 5 weeks pi, ES = 9.27, P < 0.0001; 6 weeks pi, ES = 8.87, P = 0.000332; 7 weeks pi, ES = 9.9, P = 0.0002; 8 weeks pi, ES = 5.12, P = 0.00097. P + C + A vs. vehicle: 1 week pi, ES = 24.75, P < 0.0001; 2 weeks pi, ES = 9.067, P < 0.0001; 3 weeks pi, ES = 10.84, P < 0.0001; 4 weeks pi, ES = 18.11, P = 0.00015; 5 weeks pi, ES = 11.93, P < 0.0001; 6 weeks pi, ES = 18.7, P < 0.0001; 7 weeks pi, ES = 15.61, P < 0.0001; 8 weeks pi, ES = 10.66, P = 0.000262), ^&P < 0.05 vs. PMSCs (1 week pi, ES = 24.75, P < 0.0001; 2 weeks pi, ES = 3.2, P = 0.013; 3 weeks pi, ES = 3.36, P = 0.014; 4 weeks pi, ES = 2.45, P = 0.15; 5 weeks pi, ES = 0.39, P = 0.42; 6 weeks pi, ES = 4.65, P = 0.0099; 7 weeks pi, ES = 7.39, P = 0.0001; 8 weeks pi, ES = 5.61, P = 0.0061). (B–Q) Representative immunofluorescence staining images showing infiltration of macrophages (CD68⁺) into the brain cortex and spinal cord of rats at 3 and 8 weeks pi. SC denotes transverse sections through the anterior horn of the lumbar spine, and BC denotes coronal sections of the motor cortex. Scale bar = 100 μm. (V,W) Scoring of the severity of inflammatory cell infiltration at 3 (V) and 8 weeks (W) pi. n = 5, *P < 0.05 vs. vehicle (PMSCs vs. vehicle at 3 weeks pi: ES = 17.87, P < 0.0001 in SC; ES = 17.15, P < 0.0001 in BC. P + C + A vs. vehicle at 3 weeks pi: E = 36.46, P < 0.0001 in SC; ES = 28.15, P < 0.0001 in BC. PMSCs vs. vehicle at 8 weeks pi: ES = 14.31, P < 0.0001 in SC; ES = 10.72, P < 0.0001 in BC. P + C + A vs. vehicle at 8 weeks pi: ES = 21.84, P < 0.0001 in SC; ES = 15.88, P < 0.0001 in BC), ^&P < 0.05 vs. PMSCs (3 weeks pi in SC, ES = 5.82, P = 0.0028; 3 weeks pi in BC, ES = 17.98, P < 0.0001; 8 weeks pi in SC, ES = 4.34, P = 0.017; 8 weeks pi in BC, ES = 8.87, P = 0.00058).