Figure 1
From: Diversity of innate immune cell subsets across spatial and temporal scales in an EAE mouse model
Gating strategy used for analyzing all immune cells within the CNS at steady state or during inflammatory conditions. (A) Starting from the upper left panel and going down following the arrows in dotted lines, we gated successively: CD45+ cells (R1) and human Jurkat cells (hCD3+)in R2, B cells (Bc) and NK cells (NKc) in R1, TCRαβ+ T cells in R3 (Tc, further subdivided in CD8+ and CD8− Tc), neutrophils (Ne) in R4, microglia (Mi) in R5, monocytes P1 and moDCs P2-P3 in R11 and P4-P5 macrophages in R12. Conventional DC were gated in R10 (cDC2 or CD11b+DC) and R8 (CD11b−DC or cDC1). R1 to R12 in the contour plots indicate the number of the corresponding gate. For each contour plot the represented gate is indicated on the right side above the plot. (B) Validation of microglia and monocyte-derived cell gating using bone marrow chimeric mice. Top 3 panels represent microglia (Mi). The 5 lower panels represent monocyte-derived cells (R9). For each category, the original gating (CD44-CD64 plot) is shown on the left, the recipient (CD45.1) versus donor origin of the population in the middle and the maturation status Ly-6C-MHCII plot on the right. The percentage is indicated in each gate. (A) is representative of brain at day 17 and (B) of brain at day 15.
