Figure 7

In vivo characterization of neuroimmune interactions. (A) Phagocytosis of axon debris by EYFP⁺ and EGFP⁺ cells. Phagocytic activity is associated with ramified morphology while motile cells that are non-phagocytic present elongated morphologies (n = 5). Middle panel presents phagocytic EYFP⁺ and EGFP⁺ cells either in their multicolor cellular environnement (left) or as a single channel image (right). Arrow point to phagocytic bodies. Scale bar 20 µm. (B) Quantification of the three main classes of morphologies for EGFP⁺ and EYFP⁺ cells: ameboid, elongated, ramified. (C) Evolution of the distributions of EGFP⁺ and EYFP⁺ cell morphologies during the course of the pathology (n = 5, D = post-induction day. *p < 0.05). The proportion of phagocytic (red) and motile (blue) cells is overlaid on the bar graphs.