Figure 1
Measurement of reconstitution by qPCR and flow cytometry. Adult NSG mice were injected with 1 × 106 bat bone marrow cells. (a) Detection of bat cells in bat-mice by species-specific primers. Peripheral blood samples were taken from NSG, C57BL/6, bat-mice 40-weeks post-injection, as well as bats, and assayed by qPCR with primers specific for mouse GAPDH, mouse 18S RNA, bat GAPDH and bat 18 S RNA. Data shown are the average cycle threshold (CT) values obtained from triplicates of each sample. (b–c) Forty weeks post-injection, peripheral blood from NSG (b) (n = 4) and bat-mice (c) (n = 4) were stained for CD45.1, Ter119, CD11b, CD44, MHC-II and analyzed by flow cytometry. Concatenated staining profiles are shown. (d–e) Chimerism levels in peripheral blood of adult NSG mice, ten weeks (d) and 40 weeks (e) post bat BM cell injection. Each symbol represents one mouse and the horizontal line indicates the mean value. (f) Proportions of various bat immune cell populations in bat-mice 40 weeks post-transplantation.
