Figure 3
In vivo transplantation of bat bone marrow (BM) cells with limiting dilution. Bat BM cells were isolated and injected into sub-lethally irradiated NSG pups at 5 × 103 (5 K), 1 × 104 (10 K), 5 × 104 (50 K) and 1 × 105 (100 K) cells per mouse (n = 5 for each group). Forty-weeks later, the mice were cheek bled and blood was prepared and analyzed by flow cytometry. (a) Shown is the plot of chimerism levels within the peripheral blood of individual mouse injected with indicated number of bat bone marrow cells. Each symbol represents one mouse and the horizontal line indicates the mean value. (b–d) Chimerism levels and total bat cell count in the (b) bone marrow, (c) liver and (d) spleen of individual mouse injected with indicated number of bat bone marrow cells 40 weeks post-transplantation. Each symbol represents one mouse and the horizontal line indicates the mean value. (e–h) Percentage proportion phenotypic lymphocyte subset analysis within the (e) peripheral blood, (f) bone marrow, (g) liver and (h) spleen of bat-mice injected with bat bone marrow cells.
