Figure 6

(A) Evaluation of cell viability on H-4-II-E cells after treatment with traditional and lipidated asymmetric peptide dendrimers. H-4-II-E cells were seeded at 1 × 10⁴ cells per well in 24-well plates and incubated at 37 °C in cell culture medium with 10% FBS for 24 h. Cells were exposed to FITC-siRNA (2 μg/mL), Lipofectamine 2000/FITC-siRNA (2 μg/mL) complex, or asymmetric peptide dendrimers/FITC-siRNA (2 μg/mL) complex in serum-free medium for 4 h. The complex was removed and cells incubated in 10% FBS medium for 24 h and imaged using fluorescence microscopy. Asymmetric peptide dendrimer internalization were visualized by representative bright field and fluorescence microscopy images of cells 24 h post treatment and compared to FITC-siRNA alone, Lipofectamine 2000/FITC-siRNA or Asymmetric peptide dendrimers/FITC-siRNA as indicated. Scale bar represents 200 μm. (B) Assessment of cell viability after exposure to traditional and lipidated asymmetric peptide dendrimers/siRNA complex H-4-II-E cells were exposed for 4 h to serum-free medium (no treatment), traditional (D1 to D6) and lipidated (D7 to D12) asymmetric peptide dendrimers /siRNA complex at various optimized N to P ratios, or to Lipofectamine 2000 /siRNA (0.1 pmol) complex. Cell viability was assessed after 4 h post treatment using the MTS assay. Results are expressed as percentage cell viability compared to untreated control cells and shown as mean ± S.E.M. (n=3 separate experiments), ****p<0.0001 using two way ANOVA.