Figure 8
Delivery efficiency of cationic asymmetric peptide dendrimer D9 determined using pEGFP expression. H-4-II-E cells were seeded at 1 × 104 cells per well in 24-well plates and incubated at 37 °C in cell culture medium with 10% FBS for 24 h. Cells were exposed to pEGFP (1 μg/mL), Lipofectamine 2000/pEGFP (1 μg/mL) complex, or asymmetric peptide dendrimers/pEGFP (1 μg/mL) complex in serum-free medium for 4 h. The complex was removed and cells incubated in 10% FBS medium for 48 h and imaged using fluorescence microscopy. pEGFP expressions were visualized by representative bright field and fluorescence microscopy images of cells 48 h post treatment and compared to pEGFP, Lipofectamine 2000/pEGFP or asymmetric peptide dendrimers/pEGFP as indicated. Scale bar represents 400 μm.
