Table 1 Phenotypic and genotypic characterization of the 23 isolates (20 E. coli and three E. albertii) carriers of the eae gene.

From: Impact of human-associated Escherichia coli clonal groups in Antarctic pinnipeds: presence of ST73, ST95, ST141 and ST131

Code	Serotype^a	ST^b	FG^c	Clonotype^d	Location	Origin of isolation	Virulence gene profile	eae typing	Resistance^e
EC-3	O145:H25	STnew1	A	46-C	Byers Peninsula	Southern elephant seal	fimH fimAv _MT78 iutA iucD traT eae	eae -epsilon1 (ε1)
EC-78	O145:H25	STnew1	A	46-C	Avian Island	Southern elephant seal	fimH iutA iucD traT eae	eae -epsilon1 (ε1)
EC-95	O145:H25	STnew1	A	46-C	King George Island	Southern elephant seal	fimH fimAv _MT78 iutA iucD traT eae	eae -epsilon1 (ε1)
EC-61	O8:HNM	STnew2	A	46-C	Avian Island	Southern elephant seal	fimH fimAv _MT78 iutA iucD traT eae	eae -theta1 (θ1)
EC-64	O8:HNM	STnew2	A	46-D	Avian Island	Antarctic fur seal	fimH fimAv _MT78 iutA iucD traT eae	eae -theta1 (θ1)
EC-147	O49:H10	206	A	7-E	Deception Island	Antarctic fur seal	fimH iutA iucD traT eae bfp	*eae* -kappa (k)
EC-136	O55,91:H11	29	B1	4–86	Deception Island	Antarctic fur seal	fimH cdt3 traT malX eae	eae -kappa (k)
EC-137	O55,91:H11	29	B1	4–86	Deception Island	Weddell seal	fimH cdt3 traT malX eae	eae -kappa (k)
EC-138	O55,91:H11	29	B1	4–86	Deception Island	Weddell seal	fimH cdt3 traT malX eae	eae -kappa (k)
EC-88*	O88:H25	328	B1	23–31	King George Island	Antarctic fur seal	fimH31 focG iutA iucD iroN traT eae	*eae* -beta1 (β1)
EC-90	O33:HNM	28	B2	21–90	King George Island	Southern elephant seal	fimH fimAv _MT78 cdt1 iutA iucD traT malX usp eae	eae -beta2 (β2)
EC-91	O33:HNM	28	B2	21–90	King George Island	Southern elephant seal	fimH fimAv _MT78 cdt1 iutA iucD traT malX usp eae	eae -beta2 (β2)
EC-38	O51:HNM	STnew3	B2	568-B	Ronge Island	Weddell seal	fimH cdt3 iutA iucD eae	eae -kappa (k)	NAL
EC-52	O88:HNM	STnew4	B2	568–438	Avian Island	Weddell seal	fimH cdt3 iutA iucD malX eae	eae -kappa (k)
EC-67	O51:H49	589	B2	39–135	Avian Island	Southern elephant seal	fimH iutA iucD traT eae	eae -alfa1 (α1)
EC-82	O51:H49	589	B2	39–135	Avian Island	Southern elephant seal	fimH iutA iucD traT eae	eae -alfa1 (α1)
EC-134	O51:H49	589	B2	39–135	Deception Island	Antarctic fur seal	fimH iutA iucD traT eae	eae -alfa1 (α1)
EC-148	O51:H49	589	B2	39–135	Deception Island	Antarctic fur seal	fimH iutA iucD traT tsh eae	eae -alfa1 (α1)
EC-156	O51:H49	589	B2	39–135	Deception Island	Weddell seal	fimH iutA iucD traT eae	eae -alfa1 (α1)
EC-165	O51:H49	589	B2	39–135	Deception Island	Antarctic fur seal	fimH iutA iucD traT eae	eae -alfa1 (α1)
EC-76	O84:HNM	2087	†	324-A	Avian Island	Southern elephant seal	fimH fimAv _MT78 cdt3 iutA iucD traT eae	eae -omicron (o)
EC-25	ONT:HNM	2087	†	324-A	Hannah Point	Southern elephant seal	fimH cdt3 iutA iucD traT eae	eae -omicron (o)	CEF
EC-172b	O128:HNM	STnew5	†	393–424	Deception Island	Southern elephant seal	fimH cdt3 iutA iucD traT ibeA eae	eae -kappa (k)

^aO antigen: nontypeable isolates were designated as ONT; H antigen: HNM for nonmotile isolates and HNT for those which did not react with any antisera.
^bSTs according to the Achtman scheme¹⁴; eight isolates showed new STs designated in this study as STnew1, STnew2, STnew3, STnew4 and STnew5.
^cPhylogroups established by PCR according to the Clermont et al.⁵⁶ scheme. †Escherichia albertii isolates.
^dClonotypes (fumC – fimH alleles)³⁶; nine isolates showed new fimH alleles designated as A, B, C, D and E.
^eAntimicrobial susceptibility was tested using the commercial broth microdilution minimum inhibitory concentration (MIC); the MICs were interpreted according to standard break points (CLSI, 2016); CEF: cefalotin, NAL: nalidixic acid.
*EC-88 positive for the ExPEC status.
In bold: serotypes or combinations of serotype/phylogroup/ST/intimin reported in human diarrheagenic EPEC.

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Table 1 Phenotypic and genotypic characterization of the 23 isolates (20 E. coli and three E. albertii) carriers of the eae gene.

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