Table 2 O/H, ST, plasmid and resistance profiles of six ST131 Cplx E. coli pinniped and four human isolates based on WGS analysis.

From: Impact of human-associated Escherichia coli clonal groups in Antarctic pinnipeds: presence of ST73, ST95, ST141 and ST131

Isolate code

Enterobase IDa

Oa

Ha

STa

cgSTa

wgSTa

rSTa

Sequencesb

Coveragec

Identity (%)

Inc group (pMLST)d

Resistance genese

EC-23

AM_LREC-53

O25

H4

131

30788

30659

1500

FII_1_AY458016 (RNAI-FII)

1-261/261

100.00

IncF (F2:A-:B1)

bla TEM-1C ; tet(A)

        

FIB_1_AP001918 (repFIB)

1-682/682

98.39

  

EC-24

AM_LREC-54

O25

H4

ND†

30793

30652

1500

FII_1_AY458016 (RNAI-FII)

1-261/261

100.00

IncF (F2:A-:B1)

bla TEM-1C ; tet(A)

        

FIB_1_AP001918 (repFIB)

1-682/682

98.39

  

EC-77

AM_LREC-55

O25

H4

6252

30794

30661

1503

FII_1_AY458016 (RNAI-FII)

1-261/261

95.79

IncF (F18:A-:B20*)

bla TEM-1C ; tet(A)

        

FII(29)_pUTI89_CP003035 (repFII)

1-259/259

98.46

  
        

FIB_1_AP001918 (repFIB)

1-682/682

96.77

  
        

FIC_1_AP001918 (repFIC)

1-499/499

95.59

  

EC-114

AM_LREC-56

O25

H4

131

21190

30689

1503

FIB_1_AP001918 (repFIB)

1-499/499

95.59

IncF (F18:A-:B20) + 

None

        

FIC_1_AP001918 (repFIC)

1-682/682

96.92

IncI1 (unknown ST)

 
        

I1_1_Alpha_AP005147 (RNAI-I1)

1-142/142

97.18

  

EC-160

AM_LREC-57

O25

H4

131

30821

30697

1831

FIB_1_AP001918 (repFIB)

1-682/682

96.92

IncF (F18:A-:B20)

None

        

FIC_1_AP001918 (repFIC)

1-499/499

95.59

  

EC-152

AM_LREC-58

O16

H5

131

21179

30694

1503

I1_1_Alpha_AP005147 (RNAI-I1)

1-134/142

97.01

IncI1 (unknown ST)

None

19 BA

AM_LREC-3

O25

H4

131

30799

30663

ND

FIB_1_AP001918 (repFIB)

1-682/682

98.39

IncF (F-:A-:B1)

bla TEM-1C

H1410

AM_LREC-40

O25

H4

131

21188

30715

1500

X1_4_JN935898 (repA)

1-377/377

99.20

IncX1

None

H1698

AM_LREC-41

O25

H4

131

30841

30728

1500

FII(pSFO)_AF401292 (repFII)

1-258/258

95.74

IncF (F3*:A-:B-)

None

60 SA

AM_LREC-45

O16

H5

131

30848

30731

1503

FII(pRSB107)_1_AJ851089 (repFII)

1-261/261

100.00

IncF (F1:A1:B1) + IncQ1

blaTEM-1B; strA; strB; aph(3′)-Ia; mph(A); catA1; sul2; tet(B); dfrA14

  1. aInformation from Enterobase (https://enterobase.warwick.ac.uk/species/index/ecoli): Identification (ID); O/H antigens and STs based on the 7 gene (ST), core genome (cgST), whole genome (wgST) and RNA MLST schemes predicted by Enterobase. †adk allele of EC-24 could not be predicted (ND), however, the remaining alleles matched those of ST131.
  2. b,cIn silico determination using PlasmidFinder 1.3 (https://cge.cbs.dtu.dk/services/PlasmidFinder/): bLocus targeted and GenBank accession number of the reference sequence; cNumber of query nucleotides found in the draft genome compared to the reference gene sequence deposited in the PlasmidFinder databases.
  3. dPlasmid STs identified using pMLST 1.4 (https://cge.cbs.dtu.dk/services/pMLST/).
  4. eResistome of the draft genome determined using ResFinder 2.1 (https://cge.cbs.dtu.dk/services/ResFinder/).
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