Figure 3 | Scientific Reports

Figure 3

From: Hemocytes in the extrapallial space of Pinctada fucata are involved in immunity and biomineralization

Figure 3The alternative text for this image may have been generated using AI.

Crystal-bearing granulocytes in the EPS of P. fucata. (a) Micrograph of the transparent hyalinocytes and the crystal-bearing granulocytes in different channels, 488 nm for calcein-AM, 542 nm for DiI and polar light for the crystal. The signal intensity distribution across the four-leaf clover spot was shown on the bottom. (b) Merged micrograph of 542 nm and polar light channels. (c and d) Timing diagrams of two migrating granulocytes (G1 and G2). Inset in c and d, magnification of G2 granulocyte, showing the birefringent particle in the granulocyte (white arrow head). c corresponds to 00: 56 min, and d corresponds to 00:58 min in the DeltaVision raw data. Scale bar = 10 µm. (e) Migrating speed of hyalinocytes and granulocytes in the EPS. The results represent the average of six replicates.

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