Figure 3 | Scientific Reports

Figure 3

From: Single- and two-photon imaging of human micrometastases and disseminated tumour cells with conjugates of nanobodies and quantum dots

Figure 3

Deep tissue imaging of metastatic breast tumour samples using sdAb-HER2-QDs and pAb-HER2-AF. (A) Normalized fluorescence intensity of a BT474 breast tumour section (50 µm) stained with the sdAb-HER2-QDs and imaged with either 2P-LSM (•) or confocal microscopy (). (B) Orthogonal slices of z-stacks taken from tumour sections depicted in (A) demonstrate signal intensity throughout the section (x, z-view and y, z-view) and a maximum projection of the images from the 50 µm stack when imaged at the 2P-LSM or at the confocal microscopy. Scale bar, 25 µm. (C) Normalized fluorescence intensity of two high HER2 expressing breast tumour sections (80 µm), KPL-4 and BT474, respectively stained with pAb-HER2-AF () or sdAb-HER2-QDs (•). (D) Orthogonal slices of z-stacks taken from tumour sections depicted in (C) demonstrate signal intensity throughout the section (x, z-view and y, z-view) and a maximum projection of the images from the 40 µm stack when labelled with sdAb-HER2-QDs or with the pAb-HER2-AF. Scale bar, respectively 100 µm and 50 µm.

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