Figure 5 | Scientific Reports

Figure 5

From: Gene expression analysis of human adipose tissue-derived stem cells during the initial steps of in vitro osteogenesis

Figure 5

Induced hASCs have improved adhesion capacity. The graphs in A, B and C depict the number of adherent cells obtained by the counting of the nuclei between control and induced conditions. (A) Adherent cells after 10 min of culture; (B) Adherent cells after 20 min of culture; and (C) Adherent cells after 40 min of culture. (D) Western Blot analysis and quantification of FAK protein. β-tubulin was used to normalize the amount of FAK under control and induced conditions. Representative images of a cropped Western Blot gel are displayed to evidence the bands, though uncropped images of blots are shown in Supplementary Fig. S4. ***denotes p < 0.001.

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