Figure 2
Variations in plasma choline and LPA levels obtained from whole blood with the addition of various anticoagulants. Whole blood obtained from healthy human subjects was mixed with heparin (blue), EDTA-2K (EDTA)(green), or EDTA-2K and NaF (EDTA + NaF)(red), then incubated at 37 °C (closed mark) or 4 °C (open mark) for 1 to 4 hours. After the plasma samples were collected by centrifugation at 4 °C, the concentrations of LPA (a) and choline (b) were measured in each plasma sample. (c) Eighty-micromolar choline chloride was added to whole blood with heparin, EDTA-2K (EDTA), or EDTA-2K and NaF (EDTA + NaF), then incubated at 37 °C or 4 °C for 1 to 4 hours. After the plasma samples were collected by centrifugation at 4 °C, the concentrations of choline were measured in each plasma sample. Results are given as the mean ± SD (n = 2).
