Table 2 Characteristics of murine antisera.

From: Virulence factors of Moraxella catarrhalis outer membrane vesicles are major targets for cross-reactive antibodies and have adapted during evolution

Serum

Strain Mc6

Strain Mc8

Titer1

Avidity index2

Bactericidal titer3

Titer1

Avidity index1

Bactericidal titer3

anti-Mc1

246,254

46 ± 4.2

261

255,242

34 ± 0.7

75

anti-Mc6

2,140,484

79 ± 1.4

607

751,395

53 ± 1.4

317

anti-Mc8

255,290

57 ± 0.7

234

1,001,067

43 ± 2.8

720

pre-immune

<500

ND

<3

<500

ND

<3

  1. 1The end-point of IgG ELISA titer against OMVs is defined as reciprocal value of the antiserum dilution giving an absorbance of control non-immunized mouse serum enlarged by two standard deviations (2 SD). The values are presented as geometric mean titers from two independent experiments.
  2. 2Avidity was tested by OMVs ELISA elution assay, using a chaotropic agent 1.5 M sodium thiocyanate (NaSCN) to disrupt weak antigen-antibody binding. The results are expressed as mean avidity index (AI) with SEM where AI = (OD490 with NaSCN/OD490 without NaSCN) × 100.
  3. 3The titer was defined as the reciprocal value of the highest dilution of antiserum at which ≥50% killing of the target strain was observed. The results were published in our previous paper26.
Back to article page