Figure 1
From: Transcytosis of TrkA leads to diversification of dendritic signaling endosomes
Dendritic SEs exist in multiple different endosomal populations. (A) Illustration of Imaris spot co-localization. The endosome on the bottom left of the inset is co-localized (arrow), while the other two SEs are not. (B) SEs were fixed after 2–6 hours of distal anti-FLAG antibody feeding and co-stained for endosomal markers EEA1, Rab7, and Rab11 as indicated. Arrows are co-localized, arrowheads are not. Individual channels and merged images shown. Dendrites are outlined with dotted lines. (C–E) Representative line scans with corresponding images showing intensity peaks of EEA1 (C), Rab11 (D), and Rab7 (E) in green and TrkA SEs in red. Co-extensive peaks indicate co-localization. (F) Quantification of dendritic SE co-localization with endosomal marker (n = 3–4 independent experiments, 15 fields of view per experiment).
