Figure 5
Immunomodulatory properties of MSCs from healthy (Sham donors) and diseased (OVX donors) microenvironments. MSCs were used at the 1st passage, and stimulated T cells were directly added onto MSCs for 6 h. MSC samples were then gathered for qRT-PCR analysis of the mRNA expression levels of immunomodulation marker genes Fasl (A), Hgf (B), Inos (C), Ido (D), Il10 (E), Mmp2 (F), Mmp9 (G) and Tgfβ1 (H). The relative mRNA expression levels of osteoporotic donor-derived MSCs were obtained by normalizing first against β-actin and then against those of the respective healthy donor-derived MSCs. n = 6 per group. Data are shown as mean ± SD. *P < 0.05 and **P < 0.01. NS, not significant (P > 0.05). Data were analysed using two-tailed Student’s t-test for BMMSCs or ADMSCs, respectively.
