Table 1 Primers used for RT-PCR.
From: TiO2 nanoparticles induce omphalocele in chicken embryo by disrupting Wnt signaling pathway
Gene | Gene Name | Accession number | Primer Sequence (5′-3′) |
|---|---|---|---|
GAPDH | Glyceraldehyde 3-phosphate dehydrogenase | NM_204305.1 | F:-ACTGTCAAGGCTGAGAACGG R:-ACCTGCATCTGCCCATTTGA |
HOXD13 | Homeobox D-13 | NM_205434.1 | F:-TCTGGCTAATGGCTGGAACG R:-ATCTCGGGCTGGTTTAGTGC |
CTNNB1 | ß-catenin | NM_205081.1 | F:-GTCCTGTATGAGTGGGAGCA R:-GTTTCGGGGAACATAGCAGAA |
PITX2 | Paired-like homeodomain 2 | NM_205010.1 | F:-CGATGAGTTGCATGAAGGAC R:-AGGAGGAAGGTGAGGAGGAG |
LEF1 | Lymphoid enhancer-binding factor 1 | XM_015276137.1 | F:-TCACCTACAGCGATGAGCAC R:-TATCAGGAGCTGGAGGATGC |
WNT11 | Wingless-type MMTV integration site family, member 11 | XM_015280851.1 | F:-TTCATCTTTGGCCCTGAATC R:-AGCTCGATGGATGAGCAGTT |
PRKCA | Protein kinase C | XM_004946229.2 | F:-ACAACCAGGACCTTCTGTGG R:-TCTCGTAGAGCAGCACTCCA |
CAMK2D | Calcium/calmodulin-dependent protein kinase II delta | XM_015276279.1 | F:-GCCAATCCACACCATTATCC R:-CCATCCATGTACTGCGTGAG |
ROCK1 | Rho-associated, coiled-coil containing protein kinase 1 | XM_015277931.1 | F:-TGACTGGTGGTCAGTTGGAG R:TAGAGATCTCGTTGTCATCAGG |
ROCK2 | Rho-associated, coiled-coil containing protein kinase 2 | XM_015276085.1 | F:-GACTGGTGGTCCGTAGGAGT R:-GCAGTCTCTCGGATGTTGTC |
