Figure 2 | Scientific Reports

Figure 2

From: Complement C1q stimulates the progression of hepatocellular tumor through the activation of discoidin domain receptor 1

Figure 2

C1q enhanced MAPKs signaling in HepG2 cells. After treating HepG2 cells with or without C1q for the indicated times, the expressions of the phosphorylated and total forms of p38, ERK1/2, and JNK were evaluated by western blotting (A). Bar graphs represent the following ratios; p-p38/p38 (B), p-JNK/JNK (C), and p-ERK1/2/ERK1/2 (D). Cells were pretreated with MAPKs inhibitors (20 µM each) for 1 h, then monolayers were scratched, and then treated with or without C1q. Photographs were taken after treatment for 0 or 18 h (E). The bar graph shows migration distances (F). *P < 0.05, **P < 0.01.

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