Figure 7 | Scientific Reports

Figure 7

From: Complement C1q stimulates the progression of hepatocellular tumor through the activation of discoidin domain receptor 1

Figure 7

C1q regulated epithelial-mesenchymal transition (EMT) via DDR1 in HepG2 cells. The protein levels of the EMT markers E-cadherin, vimentin, and N-cadherin were assessed in cells treated with or without C1q by western blotting. (A) Cells were treated with the indicated doses of C1q for 18 h and E-cadherin, vimentin, N-cadherin, and actin expressions were analyzed by Western blot. (B) Bar graphs of E-cadherin/Actin, (C) N-cadherin/Actin, (D) Vimentin/Actin and ratios. (E) Cells were transfected with control (siCtrl) or siDDR1 for 36 h, treated with or without C1q for 6 h, and Western blotted for E-cadherin, vimentin, N-cadherin and actin. (F) Bar graphs of E-cadherin/Actin, (G) N-cadherin/Actin, (H) Vimentin/Actin and ratios. *P < 0.05, **P < 0.01.

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