Figure 7
From: Rac1 in podocytes promotes glomerular repair and limits the formation of sclerosis
Rac1-GTP expression was not elevated at 6 h after ADR treatment. (a) S6 phosphorylation was induced at 6 h after ADR treatment, while phosphorylated PAK1 was barely observed at this time point. (b) Quantitative densitometry analysis of pS6/β–actin ratio is shown (p < 0.05). Data are presented as the mean ± SEM (n = 4). *p < 0.05. (c) Quantitative densitometry analysis of pPAK1 is shown (p = 0.73). Data are presented as the mean ± SEM (n = 3). (d) Quantitative densitometry analysis of PAK1 is shown (p = 0.60). Data are presented as the mean ± SEM (n = 3). (e) Podocyte lysates were prepared from 3 independent cultures in the absence1,2,3 or presence4,5,6 of ADR. Lysates (8 mg protein) were analysed for total Rac1 by WB analysis (Input). The same lysates (500 mg protein) were incubated with PAK1-PBD-immobilised beads, centrifuged, and washed as described in Materials and Methods. Bead-bound Rac1 (activated Rac1, Bound) was analysed by WB analysis. (f) Densitometric data for activated Rac1 precipitated with PAK1-PBD beads (n = 3).
