Figure 5
From: Inhalational Anesthetics Induce Neuronal Protein Aggregation and Affect ER Trafficking
Effect of isoflurane on the trafficking and function of CFTR. Four-hour treatment of HEK cells expressing wild-type CFTR results in a reduction of CFTR glycosylation and trafficking to the cell surface (reduced ratio of cytosolic membrane (band C) to ER (band B) CFTR populations) (left) and a reduction in the chloride channel activity of CFTR (right) (*denotes the addition of CFTR activator, #denotes the addition of CFTR inhibitor). Typical bands shown from biological triplicate experiments. All results were p < 0.05 as determined by student’s t-test between control and isoflurane exposed samples.
