Figure 3 | Scientific Reports

Figure 3

From: Ucma/GRP inhibits phosphate-induced vascular smooth muscle cell calcification via SMAD-dependent BMP signalling

Figure 3

Ucma/GRP expression is increased in calcifying mVSMCs. Cells were incubated in growth medium (CM) or osteogenic medium (OM) containing 2.6 mM phosphate. (A) RT PCR of Ucma/GRP in mouse chondrocytes and VSMCs. Bands represent several transcript variants of Ucma/GRP. (B) Immunocytochemical staining of Ucma/GRP in mouse VSMCs. Scale bars are 50 µm (top) and 20 µm (bottom) (C) QPCR quantification of Ucma/GRP expression in calcifying mVSMCs. Graph shows mean + SD, n = 4. Statistical significance was assessed using ANOVA. **p between 0.001–0.01. (D) Immunocytochemical staining of Ucma/GRP in calcifying mVSMCs. Scale bars are 100 µm.

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