Figure 4
From: An advanced human in vitro co-culture model for translocation studies across the placental barrier
Translocation of 5 µM Na-F, 5 µM FITC-dextran (40 kDa), 100 µM antipyrine, 100 µM indomethacin, 0.5 mg/ml 49 nm PS NPs and 50 µg/ml 70 nm PS NPs across each monolayer (BeWo, HPEC) and the co-cultivated membrane under static conditions. Cells were cultivated for 3 d on collagen-coated inserts before translocation studies were performed for 24 h with Na-F (a), FITC-dextran (b), 49 nm PS NP (e) and 70 nm PS NP (f) or for 6 h with antipyrine (c) and indomethacin (d). Data represent the median ± error range (upper and lower limit) of 3–4 biologically independent experiments with 1 technical replicate each.
