Figure 5
From: An advanced human in vitro co-culture model for translocation studies across the placental barrier
Seeding of the cells on the basolateral side of the insert. (a) Small rubber spacers are placed in each corner of a 6-well plate to avoid direct contact between membrane and lid. (b) 1 ml PBS is added to one well to humidify the surrounding. The reversed insert is placed into this well and 1 × 105 HPECs in 200 µl are added to the membrane. (c) The lid is closed immediately. Adhesion of the drop prevents cell aggregation in the center and medium loss. (d) The insert is placed back into a 12-well plate after 2 h incubation at 37 °C/5% CO2.
