Figure 2 | Scientific Reports

Figure 2

From: Bacteriophages are more virulent to bacteria with human cells than they are in bacterial culture; insights from HT-29 cells

Figure 2

Adsorption of phiCDHM3, phiCDHM6, and phiCDHS1 to HT-29 and HeLa cells, respectively. Phages were added onto monolayers of HT-29/HeLa cells in 24-well plates. The titres of free phages were determined at time intervals (PFU/ml). (A) phiCDHS1 and phiCDHM6 showed approximately 70%, and 40% adsorption to HT-29, respectively. While phiCDHM3 showed no noticeable drop in the number of free phages, which demonstrated no adsorption to HT-29. (B) The titres of free phages determined at time intervals remained unchanged. This established that all three phages were unable to adsorb to HeLa cells. (C) phiCDHS1 adsorption to HT-29 cells under both anaerobic and aerobic conditions. Mean values of three biological replicates are presented. Error bars denote standard error of the mean.

Back to article page