Table 2 EC50 values for oligomycin, carboxyatractyloside and acriflavin for BF 427, dKO TbMrf1 1wk and 7wk and dKO TbMrf1 + V5 TbPth4 cells that were either noninduced (−tet) or induced with tetracycline (+tet) for 2 days.

From: Cultured bloodstream Trypanosoma brucei adapt to life without mitochondrial translation release factor 1

Cell line

Oligomycin [ug/ml]

Carboxyatracty-loside [mM]

Acriflavin [nM]

BF 427

0.403 ± 0.03

>0.369

6.02 ± 0.52

dKO TbMRF1 1wk

0.006 ± 0.006

0.017 ± 0.02

2.68 ± 0.26

dKO TbMRF1 7wk

0.022 ± 0.005

0.076 ± 0.02

3.55 ± 0.17

cKO TbMrf1 7wk (+tet)

0.13 ± 0.05

0.125 ± 0.01

n.d.

T. evansi Antat 3/3

>2.657

0.009 ± 0.002

n.d.

dKO TbMRF1 7wk + V5 TbPth4 (– tet)

0.013 ± 0.03

0.027 ± 0.05

n.d.

dKO TbMRF1 7wk + V5 TbPth4 (+tet)

0.027 ± 0.01

0.06 ± 0.09

n.d.

  1. n.d. – not determined.
  2. The values represent the average and SEM of three independent experimental replicates.
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