Figure 2

Analytical gels resolving sex genotyping multiplex PCR of guinea pig tissue. (a) Concentration titration of gDNA template. Representative gel (one of triplicate experiments) of PCR products from gDNA of fixed guinea pig male pinna tissue. Dystrophin and Sry PCR products are expected to run at 212 and 135 bp respectively. Negative control, water. (b) Sex genotyping using a first deduced guinea pig Sry primer set generating a 135 bp amplicon from three (lanes 1–3), two (lanes 4–6) and one (lane 7–8) year old immunostained, archived, cochlear half turns. ML, molecular ladder. M and F are control (known) male and female genomic DNA from fixed pinna extraction. Dystrophin, 212 bp. (c) Sex genotyping PCR using a second guinea pig Sry PCR primer set, specifically derived from sequencing of the original PCR product (see Fig. 3), generates an 88 bp amplicon from one (lanes 1–2) and three (lanes 3–4) year old immunolabeled archived cochlear turns. M and F are control (known) male and female genomic DNA from fixed pinna extraction. Upper band running at 212 bp is the Dystrophin amplicon. Full-length original gel images are presented in Supplementary Information Figs 1–3.