Figure 4

Chitosan and cloxacillin combination can reduce extracellular and intracellular MAC-T infection. MAC-T cells were infected for 2 h with CNS in a MOI 10. After that, MAC-T cells were cultured in the presence of medium or different concentrations of cloxacillin [0.5, 4, 16, 64 µg/mL], chitosan [200 µg/mL] or cloxacillin and chitosan combined for 24 h. (A) Graphs bar represent bacteria viability in extracellular supernatants (CFU/mL). (B) Graphs bar represent bacteria viability in intracellular MAC-T (CFU/mL). (C) Cytokines measured in culture supernatants of MAC-T cells treated with different conditions analyzed by ELISA. These experiments were performed two independent times with three biological replicates and the data are represented by six independent replicates. Data are shown as mean ± SEM. The p values were obtained using one-way ANOVA followed by Bonferroni post-hoc analysis. P values *< 0.05, **< 0.01 and ***< 0.001 were considered significant.