Figure 6
Representative dot plots showing the localization of neutrophils (NEU) (blue dots), CD11c + (orange dots), P1 (red dots) and P2 (green dots) populations on a CD11b-F4/80 diagram in a mice injected with AA (a). Representative histograms showing the Ly6C expression in the neutrophils (blue line), CD11c + (orange line), P2 (green line) and P1 (red line) populations in the AA group (b). P1 and P2 population differ from their respective Ly6C expression. Geometric mean of Ly6C MFI expression for neutrophils (black columns), CD11C + (white columns), P2 (light grey columns) and P1 populations (dark grey columns) in CTRL (c), AA (d) and AA + αCD4 (e) groups. A significant difference in the expression of Ly6c MFI was observed between P1 and P2 populations in different conditions. In addition, a significant increase in the geometric mean of Ly6C MFI expression in the P1 population was observed after AA injection as compared to controls (data not shown). Statistical test used: Mann-Whitney. Results are expressed as the mean ± SEM, ****p < 0.0001 and **p < 0.01. Number of mice per group: AA + αCD4 n = 13; AA n = 14; CTRL n = 6.
