Table 1 Genome features of survival strains after DSB induction at IKI-L in the WT and NHEJ- contexts.

From: Genome plasticity is governed by double strand break DNA repair in Streptomyces

Strain

Conformationa

Deletion (kb)

Duplication (kb)

Insertion (kb)

Amplificationb

Predicted genome size (Mb)c

Size of the AUD (kb)

Estimated copy number

Estimated total size (kb)

WT1

Circular (6 f)

876

767

9.7

  

8.20

WT2

Circular (6c)

785

864

  

8.38

WT4

Circular (6d)

827

761

  

8.23

WT6

Circular (6e)

1,140

498

14.7

590

8,7

~16.40

WT17

Linear

481

829

  

8.65

WT18

Circular (6d)

940

701

  

8.06

∆kuA1

Circular (6 g)

984

665

  

7.98

∆kuA6

Circular (6a)

683

  

7.62

∆kuA12

Circular (6e)

1,140

498

14.7

840

12,3

~20.00

∆kuA13

Linear

481

829

  

8.65

∆kuA14

Circular (6c)

800

783

  

8.28

∆kuA17

Linear

481

829

  

8.65

∆ligCD6

Circular (6b)

1,297

18.8

  

7.02

∆ligCD7

Circular (6e)

1,097

522

32.8

325

10,7

~18.40

∆ligCD9

Circular (6e)

1,140

498

14.7

820

12,1

~20.00

∆ligCD11

Linear

481

829

  

8.65

∆ligCD16

Circular (6c)

785

864

  

8.38

∆ligCD19

Circular (6a)

2,116

  

6.18

  1. aThe chromosomal conformation referring to Fig. 6 is indicated into brackets.
  2. bAUD stands for Amplifiable unit of DNA. The copy number of the amplification was estimated considering the average coverage of sequencing reads of the amplified region relative to that of the whole genome.
  3. cThe genome size of the mutants carrying an amplification of DNA cannot be precisely evaluated.