Figure 4

Effects of transcription factor mutations on Epinephelus coioides fads2 promoter activity. Mutations of promoter deletion on SD3 (−347 to ATG) were generated according to in silico prediction, and the effects of mutation on promoter activity were compared with wild type (SD3). Promoter activity of constructs is represented on the right with the values representing normalized activity (Firefly luciferase/Renilla luciferase). Bars without sharing a common letter indicated significant differences (P < 0.05) among deletions determined by one-way ANOVA followed by Tukey’s multiple comparison test.