Table 1 Primers used for creating deletion constructs of Epinephelus coioides fads2 promoter.
Primer name | Primer sequence (5′-3′) | Fragment (bp) |
|---|---|---|
SD1-F | GGGGTACCTTTCATCTTGACCGTGTTGG | 2646 |
SD2-F | CCGGTACCGCAGACTGACTTGGCAGAGAT | 1614 |
SD3-F | GGGGTACCAATGGTAATGTGCAGTAACAC | 1137 |
SD4-F | GGGGTACCGTGGGTGAATGAGTCCGTGA | 810 |
SD5-F | GGGGTACCTGCGCCCTGCTTCACCAG | 310 |
SD-R | CCGCTCGAGCCTCACTGCTGCCTCTGG |
