Figure 2

Representative intravital image for the resection procedure of the PLN. Upper panels: images; lower panels: schema. (a) After Evans blue dye (EBD) injection into the left footpad, a 10 mm longitudinal incision was made at the popliteal fossa. (b) The PLN under the biceps femoris muscle was exposed with the aid of EBD staining. Black arrowhead; lymphatic vessel along the marginal vein. (c) Efferent and afferent lymphatic vessels, marginal vein, and blood vessels towards the PLN were ligated. (d) The PLN with connecting blood and lymphatic vessels were removed.