Figure 3

Typical image of the lymphatic flow in a normal mouse and PLN-resected mouse. ICG was injected into the footpad in (a) normal and PLN-resected mice (1 and 12 weeks after resection), and (b) PLN-resected mice (1–3 days after resection), followed by visualisation using the PDE. White, blue, and red arrows represent the PLN, ILN and ALN, respectively. The white arrowhead represents the ischial LN. (c) Immunohistochemical analysis showing the effect of PLN resection on the lymphatic vessel configuration in abdominal skin.