Figure 6

Cellular immune response to MVA-HA. Groups of mice (3 per group) were vaccinated (10⁸ PFU per mouse) with MVA-A/Can HA or MVA-A/mal/NL HA or MVA-A/Shang HA. A vector control group was vaccinated with MVA, and another control group was left untreated (naïve control). Seven days post-vaccination, all mice were euthanized and spleens harvested. Lymphocytes were cultured in vitro (10⁶ cells/well in 24-well plates) and re-stimulated by live virus infection (MOI of 1.0) with influenza A viruses. After 72 hrs incubation, supernatants were harvested, clarified, and tested by cytokine capture ELISA for secreted IFN-γ. The amount of secreted IFN-γ by cells re-stimulated with A/Can (a), A/mal/NL (b), and A/Shang (c), are shown. Each data point represents the quantity of cytokine (pg/mL) detected for each mouse minus background detected in corresponding unstimulated cells. The mean for each group is indicated by the horizontal bar.