Table 1 Oligonucleotide primers used in the amplification of HA and NA genes of A/H7 viruses.

From: Immunogenicity and Protection Against Influenza H7N3 in Mice by Modified Vaccinia Virus Ankara Vectors Expressing Influenza Virus Hemagglutinin or Neuraminidase

Name

*Oligonucleotide sequence

Description

CM-13

AGCAAAAGCAGG

Uni-12 influenza universal primer17

HA genes

nCM-1

CACCATGAACACACAGATTCTGGCTTTCA

Forward for A/Can HA

nCM-2

GCTCAAATACAGATGGTGCAGCGCAT

Reverse for A/Can HA

nCM-3

CACCATGAACACCCAGATCCTGGTGTT

Forward for A/mal/NL HA

nCM-4

GCTCAGATACAGATGGTGCAGCGCAT

Reverse for A/mal/NL HA

CM-20

CACCATGAACACTCAAATCCTGGTA

Forward for A/Shang HA

CM-21

CTTATATACAAATAGTGCACCGCAT

Reverse for A/Shang HA

NA genes

CM-54

CACCATGAATCCGAATCAGAAGATAATAACA

Forward for A/Can NA

CM-55

CTATTACTTGGGCATAAACCCAATGTT

Reverse for A/Can NA

CM-24

CACCATGAATCCAAATCAGAAGATAATAACAA

Forward for A/mal/NL NA

CM-25

GTTACTTGGGCATAAACCCAATGTT

Reverse for A/mal/NL NA

CM-22

CACCATGAATCCAAATCAGAAGATTCTA

Forward for A/Shang NA

CM-23

GGTTAGAGGAAGTACTCTATTTTAGCCCCAT

Reverse for A/Shang NA

  1. *The sequence CACC (in bold typeface) was added to the 5′ end of each forward primer to facilitate directional insertion of each PCR amplicon into the pENTR-D-TOPO vector. All oligonucleotide primers were synthesized by the Facility for Biotechnology Resources, CBER/FDA.
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