Table 4 Neuraminidase inhibiting antibody titers in MVA-vector immunized mice.

From: Immunogenicity and Protection Against Influenza H7N3 in Mice by Modified Vaccinia Virus Ankara Vectors Expressing Influenza Virus Hemagglutinin or Neuraminidase

Immune Sera

Neuraminidase Inhibition (50%) a

A/mallard/NL (N3)

A/Shanghai (N9)

A/Canada (N3)

108 MVA-mallard/NL NA

640

5

453

2 × 108 MVA-mallard/NL NA

2560

5

1280

108 MVA-Shanghai NA

5

57

14

2 × 108 MVA-Shanghai NA

5

640

14

108 MVA-Canada NA

226

5

226

2 × 108 MVA-Canada NA

3620

5

1810

  1. aNA inhibition titers were measure by ELLA as described in Materials & Methods, with MVA-N3A/mallard/NL/12/2000, H6N9 A/Shanghai/2/2016, and MVA-N3 A/Canada/rv444/2004 as sources of antigens. NA inhibition titer was defined as the reciprocal of the highest serum dilution inhibiting NA activity by ≥50%. For titer calculation, test samples with a titer <10 (below quantitation) were assigned a titer of 5. The geometric mean titers of duplicate assays of pooled serum from each treatment group are shown in the table. Homologous titers are shown in bold.
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