Figure 4
From: Functional cyclophilin D moderates platelet adhesion, but enhances the lytic resistance of fibrin
Adhesion and spreading of platelets measured by impedimetry. Impedance changes during adhesion and spreading of murine (a,b) and human (c,d) platelets. The change in impedance is represented as Cell Index (CI), a relative and dimensionless value which is calculated as CI = (Zi − Z0)/F where Zi is the impedance at an individual time point, Z0 is the impedance at the start of the experiment and F is a constant depending on the applied frequency. (a,c) Impedimetry traces: representative mean curves from 2–3 replicas. Bars represent SE. (b,d) Steepness and maximum Cell Index (CI) value were calculated from the impedimetry traces and are shown in relative units (the mean values of the steepness and maximum CI from the replicas of the vehicle control for human platelets and WT results for murine platelets were considered to be 1 for each independent set of measurements). Data are presented as means of n = 5–6, bars represent SE. Asterisks mark differences significant at P < 0.05 between the respective data pairs according to the Kolmogorov-Smirnov test. *a: P = 0.0013 *b: P = 0.0013 *c: P = 0.0013 *d: P = 0.0122 *e: P = 0.0183 *f: P = 0.0122. Abbreviations: WT, wild-type; KO, CypD−/−.
