Figure 1
From: Silent infection of human dendritic cells by African and Asian strains of Zika virus
Susceptibility of Vero and C6/36 cells towards African and Asian ZIKV strains infection. Phylogenetic analysis of the African and Asian ZIKV strains used in this study (highlighted in bold) (A). For each sequence, accession number, strain, and country and year of isolation are shown. Scale bar represents the number of substitutions per nucleotide position. Flow cytometry analysis of the frequency of ZIKV-infected Vero cells (B) 24, and 48 h p.i. and C6/36 cells (C) 24 h, and 48 h p.i. with a MOI of 0.1 TCID50/cell. Measurement by TCID50 assay of infectious ZIKV release in supernatants of Vero cultures 24 and 48 h p.i. (D) and C6/36 cultures 24, and 48 h p.i. (E) with ZIKV at a MOI of 0.1 TCID50/cell. LDH activity measurements of virus-induced cell death in supernatants of Vero (F) and C6/36 (G) cultures. Supernatant of dead cells obtained by incubating at 65 °C for 15 minutes was used as a positive control. The data shown are representative of 3 independent experiments, data are presented as mean +/− SD. Differences among strains were tested with one-way ANOVA with Tukey correction for multiple comparisons. Stars indicate significance levels; *p < 0.05, **p < 0.01, ***p < 0.001.
