Figure 5
From: Silent infection of human dendritic cells by African and Asian strains of Zika virus
IFN type I/III and ISG responses induced by MoDCs upon ZIKV infection. Fold change of gene expression levels relative to mock of IFN-β (A) and IFN-λ2/3 (B) measured by quantitative RT-PCR in MoDCs 24, and 48 h p.i. with five ZIKV strains at a MOI of 0.1 TCID50/cell and after addition of Poly(IC) and Resiquimod. Fold change of gene expression levels of selected ISGs, namely Viperin (C), MxA (D), and 2′,5′-OAS (E) in MoDCs infected with five ZIKV strains at a MOI of 0.1 TCID50/cell and after addition of Poly(IC) and Resiquimod. (F) Measurement of sfRNA to gRNA ratios by quantitative RT-PCR in MoDC cultures 24, and 48 h p.i. with five ZIKV strains at a MOI of 0.1 TCID50/cell. Data are presented as mean +/− SD of MoDCs generated from 4–12 independent blood donors. Differences among strains were tested with one-way ANOVA with Tukey correction for multiple comparisons. Stars indicate significance levels; *p < 0.05.
